Prairie Creek drains Newnans Lake, one of the largest lakes in north Florida, towards Paynes Prairie State Preserve and Orange Lake. Upland areas include flatwoods, mesic and hydric hammocks, and wetlands that include cypress swamps, depression marshes, and blackwater streams. The basin provides a corridor along an important flyway through this part of the state – annual bird surveys always find large and diverse populations of migrating and local birds. Wildlife seen recently include: Bald Eagle, Swallow-tailed Kite, Red-headed Woodpecker, Northern Bobwhite, Wild Turkey, Gopher Tortoise, American Alligator, Golden Mouse, River Otter, Florida Black Bear. There is also great floral diversity within the projects boundaries, with aquatic plants such as blue-flag iris, water lilies, lotus, pickerel week and lizard's 'tail in showy blossom in the Spring.
Prairie Creek Preserve has a well-maintained trail network that winds through a wide variety of habitat types. Areas that were previously in planted pine are in the process of being restored, and you may notice signs of prescribed fire within these areas. Management activities also include planting Longleaf pine and Wiregrass.