The 125 acre Sweetwater Preserve is located in southeast Gainesville adjacent to Paynes Prairie Preserve State Park, the Downtown Connector of the Gainesville-Hawthorne State Trail, and Boulware Springs City Park. The trailhead/entrances are located at SE 16 Avenue & Williston Road, and approximately 1300 feet north of Boulware Springs Park on the Downtown Connector of the Gainesville-Hawthorne State Trail. The preserve has approximately 3-miles of trails that are open for hiking and biking every day sunrise to sunset. Sweetwater Preserve contains a diverse assemblage of natural communities including slope forest, baygall, sinkhole lake, floodplain forest, seepage stream, xeric hammock, upland mixed forest, mesic flatwoods and sandhill.
Park at Boulware Springs and access the preserve by walking north along the GHRT. The East and West trails are connected via bridge.